Video Reflexive Ethnography (VRE) has been used for more than a decade to explore, understand and intervene in hospital work. As a methodology it has assisted social scientists to understand complex hospital team dynamics, patient involvement to achieve patient safety, and the importance of the material environment in delivering safe healthcare (Iedema et al 2013; Wyer et al 2015). Moreover, VRE has been used by clinicians to intervene, redesign and improve upon care delivery practices in ways that are bottom-up and attuned to the local needs of the team (Carroll et al 2008, Iedema et al 2009). This study builds on the VRE tradition by using VRE over a two-week period of immersion with two hospital-based teams at Mayo Clinic, USA: Breast Surgery (BS) and Frozen Section Pathology Laboratory (FSL). The two teams work in distinct spatial locales, yet their practices are intimately linked through the transfer of resected body specimens and diagnostic communications. The FSL provides rapid intra-operative assessment and decision-making regarding the presence of malignant cells at the margins of resected specimens from breast cancer patients undergoing surgery (Boughey et al., 2014, Osborn, Keeney, Jakub, Degnim, & Boughey, 2011). If the specimen margins are identified as ‘positive’ or ‘close’, surgeons can excise additional tissue at the time of initial operation, without the need for a return visit to the operating theatre (Boughey et al., 2014; Osborn, Keeney, Jakub, Degnim, & Boughey, 2011). This has achieved significantly lower reoperation rates than the US national average (Osburn 2011). Yet while the scientific and technical processes of the FSL have been described (Boughey et al., 2014; Ferreiro, Myers, & Bostwick, 1995; Gal, 2005; Osburn et al., 2011), the collaborative processes of inter-personal and human-machine interaction that constitute the production of diagnostic ‘fact’ (Latour & Woolgar, 1979) have yet to be explored and identified in these two teams. This research will report on how VRE was used collaboratively with clinicians who, through a series of five hour-long video-reflexive sessions, were enabled to “see” and “appreciate” the complex practices and spaces of “the other” in new ways. In particular, our analysis focuses on the pathology and surgical staff’s own identification of inter-team success arising from participation in video reflexivity. We will share the communicational, practice and affective elements that were identified by participants as binding the teams together in achieving successful collaboration. Mayo Clinic